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Volta V, et al.  (2005) Sen34p depletion blocks tRNA splicing in vivo and delays rRNA processing. Biochem Biophys Res Commun 337(1):89-94

Abstract: Tif6p (eIF6) is necessary for 60S biogenesis, rRNA maturation and must be released from 60S to permit 80S assembly and translation. We characterized Tif6p interactors. Tif6p is mostly on 66S-60S pre-ribosomes, partly free. Tif6p complex(es) contain nucleo-ribosomal factors and Asc1p. Surprisingly, Tif6p particle contains the low-abundance endonuclease Sen34p. We analyzed Sen34p role on rRNA/tRNA synthesis, in vivo. Sen34p depletion impairs tRNA splicing and causes unexpected 80S accumulation. Accordingly, Sen34p overexpression causes 80S decrease and increased polysomes which suggest increased translational efficiency. With delayed kinetics, Sen34p depletion impairs rRNA processing. We conclude that Sen34p is absolutely required for tRNA splicing and that it is a rate-limiting element for efficient translation. Finally, we confirm that Tif6p accompanies 27S pre-rRNA maturation to 25S rRNA and we suggest that Sen34p endonuclease in Tif6p complex may affect also rRNA maturation.

Status: Published Type: Journal Article | Research Support, Non-U.S. Gov't PubMed ID: 16188229

Topics addressed in this paper

Number of different genes curated to this paper: 35

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Topics Genes linked to topics (#1 - 10 )
ASC1 DBP9 ERB1 HAS1 LOC1 MAK11 MRT4 NIP1 NOC3 NOG1
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Topics Genes linked to topics (#11 - 20 )
NOP7 NUG1 PRT1 RLP7 RPG1 RPL11B RPL13A RPL13B RPL14B RPL16A
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Topics Genes linked to topics (#21 - 30 )
RPL16B RPL17B RPL18A RPL18B RPL20A RPL20B RPL3 RPL4A RPL5 RPL9A
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Topics Genes linked to topics (#31 - 35 )
RPL9B SEN34 SSA2 TIF6 YTM1
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