Robine N, et al. (2007) Genome-Wide Redistribution of Meiotic Double-Strand Breaks in Saccharomyces cerevisiae. Mol Cell Biol 27(5):1868-80
Abstract: Meiotic recombination is initiated by the formation of programmed DNA double-strand breaks (DSBs) catalyzed by the Spo11 protein. DSBs are not randomly distributed along chromosomes. To better understand factors that control the distribution of DSBs in budding yeast, we have examined the genome-wide binding and cleavage properties of the Gal4BD-Spo11 fusion protein. We found that Gal4BD-Spo11 cleaves only a subset of its binding sites, indicating that the association of Spo11 with chromatin is not sufficient for DSB formation. In centromere-associated regions, the centromere itself prevents DSB cleavage by tethered Gal4BD-Spo11 since its displacement restores targeted DSB formation. In addition, we observed that new DSBs introduced by Gal4BD-Spo11 inhibit surrounding DSB formation over long distances (up to 60kb), keeping constant the DSB amount per chromosomal region. Together, these results demonstrate that the targeting of Spo11 to new chromosomal locations leads both to local stimulation and genome-wide redistribution of recombination initiation and that some chromosomal regions are inherently cold regardless of Spo11 presence.
|Status: Published||Type: Journal Article||PubMed ID: 17189430|
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|Genomic co-immunoprecipitation study|
|Protein-Nucleic Acid Interactions|