Ira G, et al. (2006) Conservative inheritance of newly synthesized DNA in double-strand break-induced gene conversion. Mol Cell Biol 26(24):9424-9
Abstract: To distinguish among possible mechanisms of repair of a double-strand break (DSB) by gene conversion in budding yeast, we employed isotope density transfer to analyze budding yeast mating-type (MAT) gene switching in G2/M-arrested cells. Both newly synthesized DNA strands created during gene conversion are found at the repaired locus, leaving the donor unchanged. These results support suggestions that mitotic DSBs are primarily repaired by a Synthesis Dependent Strand Annealing (SDSA) mechanism. We also show that the proportion of crossing-over associated with DSB-induced ectopic recombination is not affected by the presence of nonhomology at one or both ends of the DSB or the presence of additional sequences that must be copied from the donor.
|Status: Published||Type: Journal Article||PubMed ID: 17030630|
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