Houghton-Larsen J and Brandt A (2006) Fermentation of High Concentrations of Maltose by Saccharomyces cerevisiae Is Limited by the COMPASS Methylation Complex. Appl Environ Microbiol 72(11):7176-82
Abstract: In Saccharomyces cerevisiae, genes encoding maltose permeases and maltases are located in the telomeric regions of different chromosomes. The COMPASS methylation complex, which methylates lysine 4 on histone H3, controls silencing of telomeric regions. Yeast strains deleted for SWD1, SWD3, SDC1, SET1, BRE2 or SPP1, encoding components of the COMPASS complex, fermented a medium containing 22% maltose with noticeably higher attenuation than the wild type, resulting in production of up to 29% more ethanol. The least effective strain was spp1. Absence of COMPASS components had no effect on fermentation of media with 20% glucose, 20% sucrose or 16% maltose. Deletion of SWD3 resulted in higher amounts of MAL12 transcript, encoding maltase, at late stages of fermentation of 22% maltose. A similar effect was seen on maltase activity and maltose uptake capability. The lysine 4 residue of histone H3 was trimethylated in wild type cells at the late stages, while only low amounts of the dimethylated form were detected. Tri- and dimethylation of this residue were not detected in strains deleted for SWD1, SWD3, SET1, BRE2 or SDC1. Trimethylated lysine 4 was only apparent at early stages (48 and 96 hours) of the fermentation in a spp1 strain. This work indicates that the COMPASS complex represses expression of maltose utilization genes during late stages of fermentation of a high concentration of maltose.
|Status: Published||Type: Journal Article||PubMed ID: 16980427|
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