Hakansson KO, et al. (2006) Crystallization of mutant forms of glutaredoxin Grx1p from yeast. Acta Crystallogr Sect F Struct Biol Cryst Commun 62(Pt 9):920-2
Abstract: Glutaredoxin Grx1p from yeast was crystallized both as an independent protein and in a protein fusion with His-tagged yellow fluorescent protein (rxYFP). A glutathionylated C30S mutant of the 12 kDa Grx1p was crystallized in two different forms in PEG 4000 at low pH. These orthorhombic and monoclinic forms diffract to 2.0 A (synchrotron radiation) and 2.7 A (rotating-anode generator), respectively. In contrast, rxYFP-Grx1p formed crystals at high pH in MgSO(4) which diffract synchrotron radiation to 2.7 A.
|Status: Published||Type: Journal Article | Research Support, Non-U.S. Gov't||PubMed ID: 16946480|
Topics addressed in this paper
- To go to the Locus page for a gene, click on the gene name.
|Protein Physical Properties|
|Protein Sequence Features|
|Protein/Nucleic Acid Structure|