SGD Paper Help


Page Contents:
Tsubota T, et al.  (2006) Double-stranded DNA Binding, an Unusual Property of DNA Polymerase {epsilon}, Promotes Epigenetic Silencing in Saccharomyces cerevisiae. J Biol Chem 281(43):32898-908

Abstract: We have previously shown that DNA polymerase epsilon (Pol epsilon) of Saccharomyces cerevisiae binds stably to double-stranded DNA (dsDNA), a property not generally associated with DNA polymerases. Here, by reconstituting Pol epsilon activity from Pol2p-Dpb2p and Dpb3p-Dpb4p, its two component subassemblies, we report that Dpb3p-Dpb4p, a heterodimer of histone-fold motif-containing subunits, is responsible for the dsDNA binding. Substitution of specific lysine residues in Dpb3p, highlighted by homology modeling of Dpb3p-Dpb4p based on the structure of the histone H2A-H2B dimer, indicated that they play roles in binding of dsDNA by Dpb3p-Dpb4p, in a manner similar to the histone-DNA interaction. The lysine-substituted dpb3 mutants also displayed reduced telomeric silencing, whose degree paralleled that of the dsDNA-binding activity of Pol epsilon in the corresponding dpb3 mutants. Furthermore, additional amino acid substitutions to lysines in Dpb4p, to compensate for the loss of positive charges in the Dpb3p mutants, resulted in simultaneous restoration of dsDNA-binding activity by Pol epsilon and telomeric silencing. We conclude that the dsDNA-binding property of Pol epsilon is required for epigenetic silencing at telomeres.

Status: Published Type: Journal Article PubMed ID: 16916794

Topics addressed in this paper

Number of different genes curated to this paper: 9

  • To find other papers on a gene and topic, click on the colored ball in the appropriate box.
  • displays other papers with information about that topic for that gene.
  • displays other papers in SGD that are associated with that topic.
    The topic is addressed in these papers but does not describe a specific gene or chromosomal feature.
  • To go to the Locus page for a gene, click on the gene name.
Topics Genes linked to topics
DPB2 DPB3 DPB4 HTA1 HTA2 HTB1 HTB2 POL2 RAD9
Additional Literature blue ball blue ball blue ball blue ball blue ball
Fungal Related Genes/Proteins blue ball blue ball blue ball blue ball blue ball blue ball
Genetic Interactions blue ball blue ball blue ball
Mutants/Phenotypes blue ball blue ball blue ball
Primary Literature blue ball blue ball blue ball blue ball
Protein Physical Properties blue ball blue ball blue ball blue ball
Protein Sequence Features blue ball blue ball blue ball blue ball blue ball blue ball
Protein-Nucleic Acid Interactions blue ball blue ball blue ball blue ball
Substrates/Ligands/Cofactors blue ball blue ball blue ball blue ball

Author Searches

To find contact information or other publications by the authors of this paper, follow these three steps:
  1. (1) Choose an author,
  2. (2) Choose a search parameter,
  3. (3) Click to implement
Page Contents: