Matecic M, et al. (2006) New alleles of SIR2 define cell-cycle-specific silencing functions. Genetics 173(4):1939-50
Abstract: The establishment of transcriptional silencing in yeast requires cell cycle progression, but the nature of this requirement is unknown. Sir2 is a protein deacetylase that is required for gene silencing in yeast. We have used temperature sensitive alleles of the SIR2 gene to assess Sir2's contribution to silencing as a function of the cell cycle. When examined in vivo these conditional alleles fall into two classes: one class exhibits a loss of silencing when raised to the non-permissive temperature regardless of cell cycle position, while the second class exhibits a mitosis-specific silencing defect. Alleles of the first class have a primary defect in protein deacetylase activity, while the class two alleles are specifically defective in Sir2-Sir4 interactions at non-permissive temperatures. Using a SIR2 temperature sensitive allele we show that silencing can be established at the HML locus during progression through the G2/M to G1 interval. These results suggest that yeast heterochromatin undergoes structural transitions as a function of the cell cycle and support the existence of a critical assembly step for silent chromatin in mitosis.
|Status: Published||Type: Journal Article||PubMed ID: 16783021|
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