SGD Paper 
Zhu XD and Sadowski PD (1998) Selection of novel, specific single-stranded DNA sequences by Flp, a duplex-specific DNA binding protein. Nucleic Acids Res 26(5):1329-36
Abstract: Flp is a member of the integrase family of site-specific recombinases. Flp is known to be a double-stranded (ds)DNA binding protein that binds sequence specifically to the 13 bp binding elements in the FRT site (Flprecognitiontarget). We subjected a random pool of oligonucleotides to the in vitro binding site selection method and have unexpectedly recovered a series of single-stranded oligonucleotides to which Flp binds with high affinity. These single-stranded oligonucleotides differ in sequence from the duplex FRT site. The minimal length of the oligonucleotides which is active is 29 nt. This single strand-specific DNA binding activity is located in the same C-terminal 32 kDa domain of Flp in which the site-specific dsDNA binding activity resides. Competition studies suggest that the apparent affinity of Flp for single-stranded oligonucleotide is somewhat less than for a complete duplex FRT site but greater than for a single duplex 13 bp binding element. We have also shown that Cre, another member of the integrase family of site-specific recombinases, also exhibits single-stranded DNA binding similar to that of Flp.
| Status: Published | Type: Journal Article | PubMed ID: 9469845 |
Topics addressed in this paper
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| Topics | Genes linked to topics |
|---|---|
| FLP1 | |
| Function/Process |  |
| Primary Literature | |
| Protein Sequence Features | |
| Protein-Nucleic Acid Interactions | |
| Substrates/Ligands/Cofactors | |
