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Friant S, et al.  (1998) Interactions between Ty1 retrotransposon RNA and the T and D regions of the tRNA(iMet) primer are required for initiation of reverse transcription in vivo. Mol Cell Biol 18(2):799-806

Abstract: Reverse transcription of the Saccharomyces cerevisiae Ty1 retrotransposon is primed by tRNA(iMet) base paired to the primer binding site (PBS) near the 5' end of Ty1 genomic RNA. The 10-nucleotide PBS is complementary to the last 10 nucleotides of the acceptor stem of tRNA(iMet). A structural probing study of the interactions between the Ty1 RNA template and the tRNA(iMet) primer showed that besides interactions between the PBS and the 3' end of tRNA(iMet), three short regions of Ty1 RNA, named boxes 0, 1, and 2.1, interact with the T and D stems and loops of tRNA(iMet). To determine if these sequences are important for the reverse transcription pathway of the Ty1 retrotransposon, mutant Ty1 elements and tRNA(iMet) were tested for the ability to support transposition. We show that the Ty1 boxes and the complementary sequences in the T and D stems and loops of tRNA(iMet) contain bases that are critical for Ty1 retrotransposition. Disruption of 1 or 2 bp between tRNA(iMet) and box 0, 1, or 2.1 dramatically decreases the level of transposition. Compensatory mutations which restore base pairing between the primer and the template restore transposition. Analysis of the reverse transcription intermediates generated inside Ty1 virus-like particles indicates that initiation of minus-strand strong-stop DNA synthesis is affected by mutations disrupting complementarity between Ty1 RNA and primer tRNA(iMet).

Status: Published Type: Journal Article PubMed ID: 9447976

Topics addressed in this paper

Number of different genes curated to this paper: 35

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Topics Genes linked to topics (#1 - 10 )
IMT1 IMT2 IMT3 IMT4 SPT3 YARCTy1-1 YBLWTy1-1 YBRWTy1-2 YDRCTy1-1 YDRCTy1-2
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
DNA/RNA Sequence Features blue ball blue ball blue ball blue ball blue ball blue ball
Mutants/Phenotypes blue ball
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Topics Genes linked to topics (#11 - 20 )
YDRCTy1-3 YDRWTy1-4 YDRWTy1-5 YERCTy1-1 YERCTy1-2 YGRCTy1-2 YGRCTy1-3 YGRWTy1-1 YHRCTy1-1 YJRWTy1-1
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
DNA/RNA Sequence Features blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Nucleic Acid Interaction blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Other Features blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball

Topics Genes linked to topics (#21 - 30 )
YJRWTy1-2 YLRCTy1-1 YLRWTy1-2 YLRWTy1-3 YMLWTy1-1 YMLWTy1-2 YMRCTy1-3 YMRCTy1-4 YNLCTy1-1 YNLWTy1-2
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
DNA/RNA Sequence Features blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Nucleic Acid Interaction blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Other Features blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#31 - 35 )
YOLWTy1-1 YORWTy1-2 YPLWTy1-1 YPRCTy1-2 YPRCTy1-4
Additional Literature blue ball blue ball blue ball blue ball blue ball
DNA/RNA Sequence Features blue ball blue ball blue ball blue ball blue ball
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