Faber AW, et al. (2006) 5'-End formation of yeast 5.8S(L) rRNA is an endonucleolytic event. Biochem Biophys Res Commun 345(2):796-802
Abstract: Like most eukaryotes, Saccharomyces cerevisiae cells contain a minor 5.8S(L) rRNA that, relative to the major 5.8S(S) species, carries several extra nucleotides at the 5'-end. The two species are produced by alternative pathways that differ in the events removing the 3'-terminal region of Internal Transcribed Spacer 1 from the 27SA2 pre-rRNA. Whereas the pathway leading to 5.8S(S) rRNA is well established, that producing the 5'-end of 5.8S(L) (called B1(L)) is poorly understood. Northern analysis of two different mutants of S. cerevisiae that overproduce 5.8S(L) rRNA revealed the presence of a fragment corresponding to the 3'-terminal region of Internal Transcribed Spacer 1 (ITS1) directly upstream from site B1(L). Immunoprecipitation experiments showed this fragment to be associated with the trans-acting factor Rrp5p required for processing at the early sites A0-A3. Together these data clearly support that the 5'-end of 5.8S(L) rRNA is an endonucleolytic event. In vivo mutational analysis demonstrated the lack of any cis-acting sequence elements directing this cleavage within ITS1.
|Status: Published||Type: Journal Article||PubMed ID: 16701559|
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