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Fleischer TC, et al.  (2006) Systematic identification and functional screens of uncharacterized proteins associated with eukaryotic ribosomal complexes. Genes Dev 20(10):1294-307

Abstract: Translation regulation is a critical means by which cells control growth, division, and apoptosis. To gain further insight into translation and related processes, we performed multifaceted mass spectrometry-based proteomic screens of yeast ribosomal complexes and discovered an association of 77 uncharacterized yeast proteins with ribosomes. Immunoblotting revealed an EDTA-dependent cosedimentation with ribosomes in sucrose gradients for 11 candidate translation-machinery-associated (TMA) proteins. Tandem affinity purification linked one candidate, LSM12, to the RNA processing proteins PBP1 and PBP4. A second candidate, TMA46, interacted with RBG1, a GTPase that interacts with ribosomes. By adapting translation assays to high-throughput screening methods, we showed that null yeast strains harboring deletions for several of the TMA genes had alterations in protein synthesis rates (TMA7 and TMA19), susceptibility to drugs that inhibit translation (TMA7), translation fidelity (TMA20), and polyribosome profiles (TMA7, TMA19, and TMA20). TMA20 has significant sequence homology with the oncogene MCT-1. Expression of human MCT-1 in the Deltatma20 yeast mutant complemented translation-related defects, strongly implying that MCT-1 functions in translation-related processes. Together these findings implicate the TMA proteins and, potentially, their human homologs, in translation related processes.

Status: Published Type: Journal Article PubMed ID: 16702403

Topics addressed in this paper

Number of different genes curated to this paper: 81

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Topics Topics not linked to Genes Genes linked to topics (#1 - 10 )
AIM14 AIM21 ALB1 ALE1 APE4 ARB1 BCH1 DUG1 ESL2 FRD1
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Topics Genes linked to topics (#11 - 20 )
GDE1 GET4 GIP3 GVP36 HEL2 HER1 IMA1 LAA1 LSM12 MDY2
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Topics Genes linked to topics (#21 - 30 )
MPP6 MRPS12 MTC1 NBA1 NSI1 NVJ2 OTU2 PBP1 PBP4 PML39
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Topics Genes linked to topics (#31 - 40 )
POM33 RBG1 RKR1 RSA4 RTC1 SEG1 SKP2 SYH1 TAE2 TMA10
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Topics Genes linked to topics (#41 - 50 )
TMA108 TMA16 TMA17 TMA19 TMA20 TMA22 TMA23 TMA46 TMA64 TMA7
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Topics Genes linked to topics (#51 - 60 )
TVP18 VMR1 YAL064W YBL028C YDR186C YDR239C YEL043W YET1 YET2 YGR054W
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Topics Genes linked to topics (#61 - 70 )
YGR242W YGR283C YHR020W YHR080C YJL028W YJL118W YJL213W YKL222C YLL032C YMR114C
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Topics Genes linked to topics (#71 - 80 )
YMR226C YMR295C YNL208W YNL247W YOL099C YOR019W YPL073C YPL225W YPP1 YPR096C
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Topics Genes linked to topics (#81 )
YPR148C
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