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Butcher RA, et al.  (2006) Microarray-based method for monitoring yeast overexpression strains reveals small-molecule targets in TOR pathway. Nat Chem Biol 2(2):103-9

Abstract: Identification of the cellular targets of small-molecule hits in phenotypic screens is a central challenge in the development of small molecules as biological tools and potential therapeutics. To facilitate the process of small-molecule target identification, we developed a global, microarray-based method for monitoring the growth of pools of yeast strains, each overexpressing a different protein, in the presence of small molecules. Specifically, the growth of Saccharomyces cerevisiae strains harboring approximately 3,900 different overexpression plasmids was monitored in the presence of rapamycin, which inhibits the target of rapamycin (TOR) proteins. TOR was successfully identified as a candidate rapamycin target, and many additional gene products were implicated in the TOR signaling pathway. We also characterized the mechanism of LY-83583, a small-molecule suppressor of rapamycin-induced growth inhibition. These data enabled functional links to be drawn between groups of genes implicated in the TOR pathway, identified several candidate targets for LY-83583, and suggested a role for mitochondrial respiration in mediating rapamycin sensitivity.

Status: Published Type: Journal Article PubMed ID: 16415861

Topics addressed in this paper

Number of different genes curated to this paper: 58

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Topics Topics not linked to Genes Genes linked to topics (#1 - 10 )
BMH1 BRE5 BUD27 BUD8 CAJ1 DCR2 DGK1 ELP2 FET4 FPR1
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Genetic Interactions blue ball
Large-scale phenotype analysis yg ball
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Topics Genes linked to topics (#11 - 20 )
FRE5 GEP3 GIC2 GIS4 GSH1 GUF1 HFI1 HLR1 HMI1 HOL1
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Topics Genes linked to topics (#21 - 30 )
HOS1 HSP104 IMA1 IMA2 IMA4 IML2 IRC8 MAM1 MDL1 MRPS28
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Topics Genes linked to topics (#31 - 40 )
NAM7 NCL1 PDR16 PDR3 PET111 PPG1 PRM6 RIM11 RTT106 SGN1
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Topics Genes linked to topics (#41 - 50 )
SLX4 SMA2 SSE1 SUV3 TLG2 TOR1 TPO4 TPO5 UPF3 YDR222W
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Topics Genes linked to topics (#51 - 58 )
YDR514C YKR075C YMD8 YMR034C YMR102C YRM1 YRR1 ZDS2
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Mutants/Phenotypes blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Techniques and Reagents blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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