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Carroll CW and Morgan DO  (2005) Enzymology of the anaphase-promoting complex. Methods Enzymol 398:219-30

Abstract: The anaphase-promoting complex (APC) is an ubiquitin-protein ligase that promotes mitotic progression by catalyzing the ubiquitination of numerous proteins, including securin and cyclin. Its complex subunit composition and extensive regulation make the APC an active subject of investigation for both cell biologists and enzymologists. This chapter describes a system for the reconstitution and quantitative analysis of APC activity from budding yeast in vitro. We focus in particular on the measurement of processive ubiquitination, which complements traditional analysis of the reaction rate as a means to elucidate the molecular details of substrate recognition and ubiquitination by the APC.

Status: Published Type: Journal Article PubMed ID: 16275331

Topics addressed in this paper

Number of different genes curated to this paper: 16

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Topics Genes linked to topics (#1 - 10 )
AMA1 APC1 APC11 APC2 APC4 APC5 APC9 CDC16 CDC20 CDC23
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein-protein Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Techniques and Reagents blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball

Topics Genes linked to topics (#11 - 16 )
CDC26 CDC27 CDH1 DOC1 MND2 SWM1
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball
Protein-protein Interactions blue ball blue ball blue ball blue ball blue ball blue ball
Techniques and Reagents blue ball blue ball blue ball blue ball blue ball blue ball

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