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Hara A, et al.  (1999) Construction of an autonomously replicating plasmid in n-alkane-assimilating yeast, Candida tropicalis. J Biosci Bioeng 87(6):717-20

Abstract: A transformation system using the autonomously replicating plasmid in the n-alkane-assimilating and asporogenic diploid yeast, Candida tropicalis, was developed. For the cloning of a DNA fragment containing a potential autonomously replicating sequence (ARS) from the genomic DNA of C. tropicalis, the ura3 mutant obtained using ethylmethane sulfonate as the host and the URA3 gene amplified by PCR using the C. tropicalis genomic DNA as a selectable marker were prepared. Comparison of ARSs among yeasts revealed that the consensus sequence found in S. cerevisiae was also present in C. tropicalis. The autonomously replicating plasmid containing the putative ARS as the shuttle vector, capable of replicating in both E. coli and C. tropicalis, was first constructed. The transformation system using this plasmid, in addition to the integrative transformation system, will be applicable to genetic studies of C. tropicalis.

Status: Published Type: Journal Article PubMed ID: 16232544

Topics addressed in this paper

Number of different genes curated to this paper: 83

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Topics Genes linked to topics (#1 - 10 )
ARS1001 ARS1002 ARS1003 ARS1004 ARS1005 ARS1006 ARS1007 ARS1008 ARS1009 ARS101
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Fungal Related Genes/Proteins blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball

Topics Genes linked to topics (#11 - 20 )
ARS1010 ARS1011 ARS1012 ARS1013 ARS1014 ARS1015 ARS1016 ARS1017 ARS1018 ARS1019
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Fungal Related Genes/Proteins blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball

Topics Genes linked to topics (#21 - 30 )
ARS1020 ARS1021 ARS1022 ARS1023 ARS1024 ARS1025 ARS1411 ARS1412 ARS1413 ARS1414
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Fungal Related Genes/Proteins blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#31 - 40 )
ARS1501 ARS1502 ARS300 ARS301 ARS302 ARS303 ARS304 ARS305 ARS306 ARS307
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Fungal Related Genes/Proteins blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball

Topics Genes linked to topics (#41 - 50 )
ARS308 ARS309 ARS310 ARS313 ARS314 ARS315 ARS316 ARS317 ARS318 ARS319
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Fungal Related Genes/Proteins blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball

Topics Genes linked to topics (#51 - 60 )
ARS320 ARS502 ARS503 ARS504 ARS507 ARS508 ARS510 ARS511 ARS512 ARS513
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Fungal Related Genes/Proteins blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#61 - 70 )
ARS514 ARS515 ARS516 ARS517 ARS518 ARS519 ARS520 ARS521 ARS522 ARS523
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Fungal Related Genes/Proteins blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball

Topics Genes linked to topics (#71 - 80 )
ARS601 ARS602 ARS603 ARS603.5 ARS604 ARS605 ARS606 ARS607 ARS608 ARS609
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Fungal Related Genes/Proteins blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball

Topics Genes linked to topics (#81 - 83 )
ARS701 ARS901 URA3
Additional Literature blue ball blue ball blue ball
Fungal Related Genes/Proteins blue ball blue ball blue ball
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