---

Shimoi H, et al.  (2000) Molecular cloning and application of a gene complementing pantothenic acid auxotrophy of sake yeast kyokai no. 7. J Biosci Bioeng 90(6):643-7

Abstract: Kyokai no. 7 is the most widely used yeast in sake brewing. This yeast is a pantothenic acid auxotroph at 35 degrees C, and this phenotype has been used to distinguish Kyokai no. 7 from other sake yeasts. We cloned a DNA fragment complementing the pantothenic acid auxotrophy from a genomic library of a Saccharomyces cerevisiae laboratory strain. DNA sequence analysis revealed that the DNA fragment encodes ECM31, the deletion of which had previously been identified as a calcofluor white-sensitive mutation. The ECM31 product is similar to the Escherichia coli ketopantoate hydroxymethyltransferase. Disruption of ECM31 in a laboratory S. cerevisiae strain resulted in pantothenic acid auxotrophy, indicating that ECM31 is also involved in pantothenic acid synthesis in yeast. A hybrid of a Kyokai no. 7 haploid and the ecm31 disruptant required pantothenic acid at 35 degrees C for its growth, suggesting that Kyokai no. 7 possesses a temperature-sensitive allele of ECM31. Thus, the ECM31 gene can be used as a selective marker in the transformation of Kyokai no. 7.

Status: Published

Type: Journal Article

PubMed ID: 16232925

---

Author Searches

To find contact information or other publications by the authors of this paper, follow these three steps:

1. (1) Choose an author,
2. (2) Choose a search parameter,
3. (3) Click to implement

Shimoi H Okuda M Ito K Papers in SGD Colleagues in SGD PubMed Google Scholar

---