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Lutzmann M, et al.  (2005) Reconstitution of Nup157 and Nup145N into the Nup84 complex. J Biol Chem 280(18):18442-51

Abstract: About 30 different nucleoporins (Nups) constitute the nuclear pore complex. We have affinity-purified 28 of these nuclear pore proteins and identified new nucleoporin interactions by this analysis. We found that Nup157 and Nup170, two members of the large structural Nups, and the Gly-Leu-Phe-Gly nucleoporin Nup145N specifically co-purified with members of the Nup84 complex. In addition, Nup145N co-enriched during Nup157 purification. By in vitro reconstitution, we demonstrate that Nup157 and Nup145N form a nucleoporin subcomplex. Moreover, we show that Nup157 and Nup145N bind to the heptameric Nup84 complex. This assembly thus represents approximately one-third of all nucleoporins. To characterize Nup157 structurally, we purified and analyzed it by electron microscopy. Nup157 is a hollow sphere that resembles a clamp or a gripping hand. Thus, we could reconstitute an interaction between a large structural Nup, an FG repeat Nup, and a major structural module of the nuclear pore complex.

Status: Published Type: Journal Article PubMed ID: 15741174

Topics addressed in this paper

Number of different genes curated to this paper: 21

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Topics Genes linked to topics (#1 - 10 )
GLE2 KAP123 MEX67 MLP1 NIC96 NSP1 NUP116 NUP120 NUP133 NUP145
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Topics Genes linked to topics (#11 - 20 )
NUP157 NUP159 NUP170 NUP49 NUP57 NUP82 NUP84 NUP85 SAC3 SEC13
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Protein Physical Properties blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#21 )
SEH1
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Protein Physical Properties blue ball
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