SGD Paper 
Yuasa N, et al. (2005) Two Alleles of the Sulfite Resistance Genes Are Differentially Regulated in Saccharomyces cerevisiae. Biosci Biotechnol Biochem 69(8):1584-8
Abstract: The sulfite resistance gene, SSU1-R, is widely distributed in wine yeasts. This gene has an upstream region distinct from that of the allelic gene, SSU1 and SSU1-R is expressed at a much higher level than SSU1. We characterized the promoters of both of these genes by analysis of their activity using the LacZ gene as a reporter. FZF1, the activator gene of SSU1, was shown to regulate SSU1-R expression indirectly. SSU1-R expression was activated under microaerobic conditions, and four 76-bp repeats, present within the SSU1-R promoter region, was essential for high expression. These results indicate that SSU1-R expression is regulated in different manner from that of SSU1. By deletion analysis of the SSU1-R promoter region, we found that at least two of the 76-bp repeats are necessary for promoter activity, and that the number of 76-bp repeats influences the activity. Hence, it was suggested that the number of 76-bp repeats increases in wine yeasts that require strong sulfite resistance.
| Status: Published | Type: Journal Article | PubMed ID: 16116289 |
Topics addressed in this paper
Number of different genes curated to this paper: 2
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