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Courel M, et al.  (2005) Direct activation of genes involved in intracellular iron use by the yeast iron-responsive transcription factor Aft2 without its paralog Aft1. Mol Cell Biol 25(15):6760-71

Abstract: The yeast Saccharomyces cerevisiae contains a pair of paralogous iron-responsive transcription activators, Aft1 and Aft2. Aft1 activates the cell surface iron uptake systems in iron depletion, while the role of Aft2 remains poorly understood. This study compares the functions of Aft1 and Aft2 in regulating the transcription of genes involved in iron homeostasis, with reference to the presence/absence of the paralog. Cluster analysis of DNA microarray data identified the classes of genes regulated by Aft1 or Aft2, or both. Aft2 activates the transcription of genes involved in intracellular iron use in the absence of Aft1. Northern blot analyses, combined with chromatin immunoprecipitation experiments on selected genes from each class, demonstrated that Aft2 directly activates the genes SMF3 and MRS4 involved in mitochondrial and vacuolar iron homeostasis, while Aft1 does not. Computer analysis found different cis-regulatory elements for Aft1 and Aft2, and transcription analysis using variants of the FET3 promoter indicated that Aft1 is more specific for the canonical iron-responsive element TGCACCC than is Aft2. Finally, the absence of either Aft1 or Aft2 showed an iron-dependent increase in the amount of the remaining paralog. This may provide additional control of cellular iron homeostasis.

Status: Published Type: Journal Article PubMed ID: 16024809

Topics addressed in this paper

Number of different genes curated to this paper: 52

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Topics Topics not linked to Genes Genes linked to topics (#1 - 10 )
AFT1 AFT2 AHP1 AKR1 APE1 ARA2 ARN1 ARN2 ATG34 ATX1
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DNA/RNA Sequence Features blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Function/Process blue ball blue ball
Genetic Interactions blue ball blue ball
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Omics yg ball
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Topics Genes linked to topics (#11 - 20 )
BIO5 BNA2 CAD1 CCC2 COT1 ECL1 ECM4 ENB1 FET3 FET5
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DNA/RNA Sequence Features blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#21 - 30 )
FIT1 FIT2 FIT3 FRE1 FRE2 FRE3 FRE4 FRE5 FRE6 FTH1
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DNA/RNA Sequence Features blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#31 - 40 )
FTR1 GRX4 HCR1 HMX1 HSP26 ISU1 MRS4 NFT1 OYE3 PEP4
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Topics Genes linked to topics (#41 - 50 )
PRB1 SIT1 SMF3 TIS11 UBC8 VHT1 VMR1 YDL124W YMR034C YOR387C
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Topics Genes linked to topics (#51 - 52 )
ZPS1 ZRT1
Additional Literature blue ball blue ball
DNA/RNA Sequence Features blue ball blue ball
Regulation of blue ball blue ball
Transcription blue ball blue ball
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