SGD Paper Help


Page Contents:
Stribinskis V, et al.  (2005) Rpm2p, a component of yeast mitochondrial RNase P, acts as a transcriptional activator in the nucleus. Mol Cell Biol 25(15):6546-58

Abstract: Rpm2p, a protein subunit of yeast mitochondrial RNase P, has another function that is essential in cells lacking the wild-type mitochondrial genome. This function does not require the mitochondrial leader sequence and appears to affect transcription of nuclear genes. Rpm2p expressed as a fusion protein with green fluorescent protein localizes to the nucleus and activates transcription from promoters containing lexA-binding sites when fused to a heterologous DNA binding domain, lexA. The transcriptional activation region of Rpm2p contains two leucine zippers that are required for transcriptional activation and are conserved in the distantly related yeast Candida glabrata. The presence of a mitochondrial leader sequence does not prevent a portion of Rpm2p from locating to the nucleus, and several observations suggest that the nuclear location and transcriptional activation ability of Rpm2p are physiologically significant. The ability of RPM2 alleles to suppress tom40-3, a temperature-sensitive mutant of a component of the mitochondrial import apparatus, correlates with their ability to transactivate the reporter genes with lexA-binding sites. In cells lacking mitochondrial DNA, Rpm2p influences the levels of TOM40, TOM6, TOM20, TOM22, and TOM37 mRNAs, which encode components of the mitochondrial import apparatus, but not that of TOM70 mRNA. It also affects HSP60 and HSP10 mRNAs that encode essential mitochondrial chaperones. Rpm2p also increases the level of Tom40p, as well as Hsp60p, but not Atp2p, suggesting that some, but not all, nucleus-encoded mitochondrial components are affected.

Status: Published Type: Journal Article PubMed ID: 16024791

Topics addressed in this paper

Number of different genes curated to this paper: 9

  • To find other papers on a gene and topic, click on the colored ball in the appropriate box.
  • displays other papers with information about that topic for that gene.
  • displays other papers in SGD that are associated with that topic.
    The topic is addressed in these papers but does not describe a specific gene or chromosomal feature.
  • To go to the Locus page for a gene, click on the gene name.
Topics Genes linked to topics
HSP10 HSP60 RPM2 SAM37 SEF1 TOM20 TOM22 TOM40 TOM6
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Cellular Location blue ball
Function/Process blue ball blue ball
Fungal Related Genes/Proteins blue ball
Mutants/Phenotypes blue ball
Primary Literature blue ball
Protein Sequence Features blue ball
Regulation of blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Regulatory Role blue ball blue ball
RNA Levels and Processing blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Strains/Constructs blue ball

Author Searches

To find contact information or other publications by the authors of this paper, follow these three steps:
  1. (1) Choose an author,
  2. (2) Choose a search parameter,
  3. (3) Click to implement
Page Contents: