SGD Paper Help


Page Contents:
Zhu Y, et al.  (2005) Gpi17p does not stably interact with other subunits of glycosylphosphatidylinositol transamidase in Saccharomyces cerevisiae. Biochim Biophys Acta 1735(1):79-88

Abstract: Homologues of Gpi8p, Gaa1p, Gpi16p, Gpi17p, and Cdc91p are essential components of the GPI transamidase complex that adds glycosylphosphatidylinositols (GPIs 1) to newly synthesized proteins in the ER. In mammalian cells, these five subunits remain stably associated with each other in detergent. In yeast, we find no stable stoichiometric association of Gpi17p with the Gpi8p-Gpi16p-Gaa1p core in detergent extracts. Random and site-directed mutagenesis generated mutations in several highly conserved amino acids but did not yield nonfunctional alleles of Gpi17p and a saturating screen did not yield any dominant negative alleles of Gpi17p. Moreover, Gpi8p becomes unstable when any one of the other subunits is depleted, whereas Gpi17p is slightly affected only by the depletion of Gaa1p. These data suggest that yeast Gpi17p may be able to exert its GPI anchoring function without interacting in a stable and continuous manner with the other GPI-transamidase subunits. Shutting down ER-associated and vacuolar protein degradation pathways has no effect on the levels of Gpi17p or other transamidase subunits.

Status: Published Type: Journal Article PubMed ID: 15939668

Topics addressed in this paper

Number of different genes curated to this paper: 4

  • To find other papers on a gene and topic, click on the colored ball in the appropriate box.
  • displays other papers with information about that topic for that gene.
  • displays other papers in SGD that are associated with that topic.
    The topic is addressed in these papers but does not describe a specific gene or chromosomal feature.
  • To go to the Locus page for a gene, click on the gene name.
Topics Genes linked to topics
GAA1 GPI16 GPI17 GPI8
Cellular Location blue ball blue ball blue ball blue ball
Fungal Related Genes/Proteins blue ball
Mutants/Phenotypes blue ball blue ball blue ball blue ball
Non-Fungal Related Genes/Proteins blue ball
Primary Literature blue ball blue ball blue ball blue ball
Protein Processing/Modification/Regulation blue ball blue ball blue ball blue ball
Protein Sequence Features blue ball
Protein-protein Interactions blue ball blue ball blue ball blue ball
Strains/Constructs blue ball blue ball blue ball blue ball
Techniques and Reagents blue ball blue ball blue ball blue ball

Author Searches

To find contact information or other publications by the authors of this paper, follow these three steps:
  1. (1) Choose an author,
  2. (2) Choose a search parameter,
  3. (3) Click to implement
Page Contents: