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Millson SH, et al.  (2005) A two-hybrid screen of the yeast proteome for Hsp90 interactors uncovers a novel Hsp90 chaperone requirement in the activity of a stress-activated mitogen-activated protein kinase, Slt2p (Mpk1p). Eukaryot Cell 4(5):849-60

Abstract: The Hsp90 chaperone cycle catalyzes the final activation step of several important eukaryotic proteins (Hsp90 "clients"). Although largely a functional form of Hsp90, an Hsp90-Gal4p DNA binding domain fusion (Hsp90-BD) displays no strong interactions in the yeast two-hybrid system, consistent with a general transience of most Hsp90-client associations. Strong in vivo interactions are though detected when the E33A mutation is introduced into this bait, a mutation that should arrest Hsp90-client complexes at a stage where the client is stabilized, yet prevented from attaining its active form. This E33A mutation stabilized the two-hybrid interactions of the Hsp90-BD fusion with approximately 3% of the Saccharomyces cerevisiae proteome in a screen of the 6,000 yeast proteins expressed as fusions to the Gal4p activation domain (AD). Among the detected interactors were the two stress-activated mitogen-activated protein (MAP) kinases of yeast, Hog1p and Slt2p (Mpk1p). Column retention experiments using wild-type and mutant forms of Hsp90 and Slt2p MAP kinase, as well as quantitative measurements of the effects of stress on the two-hybrid interaction of mutant Hsp90-BD and AD-Slt2p fusions, revealed that Hsp90 binds exclusively to the dually Thr/Tyr-phosphorylated, stress-activated form of Slt2p [(Y-P,T-P)Slt2p] and also to the MAP kinase domain within this (Y-P,T-P)Slt2p. Phenotypic analysis of a yeast mutant that expresses a mutant Hsp90 (T22Ihsp82) revealed that Hsp90 function is essential for this (Y-P,T-P)Slt2p to activate one of its downstream targets, the Rlm1p transcription factor. The interaction between Hsp90 and (Y-P,T-P)Slt2p, characterized in this study, is probably essential in this Hsp90 facilitation of the Rlm1p activation by Slt2p.

Status: Published Type: Journal Article PubMed ID: 15879519

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ACF4 ACT1 AGP2 AHA1 AIM2 AIM37 ANT1 AQR1 ARE1 ARE2
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Large-scale protein interaction yg ball
Omics yg ball
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ARG4 ARR3 ASG7 ATG14 ATG16 ATG32 AVT6 BAP3 BNA3 BOS1
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BRL1 BSC6 BSD2 BSP1 CAM1 CAN1 CAR2 CCT4 CDC12 CDC37
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Topics Genes linked to topics (#31 - 40 )
CFD1 CNS1 COS3 COS6 COS7 DAL5 DCG1 DDR2 DET1 DPS1
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ECI1 EPT1 ERV46 ESBP6 FLD1 FRS2 FRT2 FUN26 GCN2 GDA1
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Topics Genes linked to topics (#51 - 60 )
GMH1 GND1 GRX5 GVP36 HAM1 HAP1 HNT1 HOG1 HSP10 HSP42
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Topics Genes linked to topics (#61 - 70 )
HSP60 HSP82 HTB1 HTS1 HXT1 HXT10 HXT17 HXT5 INP54 IZH4
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Topics Genes linked to topics (#71 - 80 )
KSS1 LAC1 LYP1 LYS21 MAL11 MCX1 MDH3 MET14 MIM1 MKK1
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Topics Genes linked to topics (#81 - 90 )
MNT3 MOG1 MUP1 MUP3 NET1 NMD5 NOP15 NUP57 OAF3 OLE1
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Topics Genes linked to topics (#91 - 100 )
PDR3 PDX3 PEP12 PEX11 PEX34 PFA3 PHM6 PHO86 POS5 PRM4
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Topics Genes linked to topics (#101 - 110 )
PUT2 PUT4 PYK2 QDR1 RAS1 RBD2 RDS1 RGC1 RIM1 RLM1
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Topics Genes linked to topics (#111 - 120 )
RPL18A RRD2 RRP17 SAP30 SBA1 SFT1 SGM1 SHE3 SHE4 SHO1
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Protein-protein Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball

Topics Genes linked to topics (#121 - 130 )
SIR2 SLT2 SMA2 SOR1 SOR2 SRF1 SRT1 SSE1 STE11 STI1
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Topics Genes linked to topics (#131 - 140 )
STS1 TAH1 THI13 TLG2 TOM22 TPM1 TPO5 TSC13 TVP23 UBC6
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Topics Genes linked to topics (#141 - 150 )
UBX7 UGP1 UIP4 UIP5 VMA4 VPS55 WHI5 WTM2 XYL2 YBR220C
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Topics Genes linked to topics (#151 - 160 )
YCK1 YCR101C YDJ1 YDL012C YDL180W YDL199C YER156C YFT2 YIR042C YJL114W
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Topics Genes linked to topics (#161 - 170 )
YKL069W YLR036C YML131W YMR155W YNL194C YOL029C YOR097C YPL014W YPL109C YPL199C
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Primary Literature blue ball blue ball blue ball
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Topics Genes linked to topics (#171 - 179 )
YPL257W YPL264C YPR003C YPR089W YPR127W YPR148C YPT31 YPT53 YPT7
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