SGD Paper Help


Page Contents:
Liao XH, et al.  (2005) Characterization of recombinant Saccharomyces cerevisiae telomerase core enzyme purified from yeast. Biochem J 390(Pt 1):169-76

Abstract: Telomerase is a cellular reverse transcriptase that elongates the single-stranded chromosome ends and oligonucleotides in vivo and in vitro. In Saccharomyces cerevisiae, Est2p (telomerase catalytic subunit) and Tlc1 (telomerase RNA template subunit) constitute the telomerase core complex. We co-overexpressed GST-Est2p and Tlc1 in Saccharomyces cerevisiae, and reconstituted the telomerase activity. The GST-Est2p/Tlc1 complex was partially purified by ammonium sulfate fraction and affinity chromatography on Glutathione beads, and the partially purified telomerase didn't contain the other two subunits of telomerase holoenzyme, Est1p and Est3p. The purified recombinant GST-Est2p/Tlc1 telomerase core complex could specifically add nucleotides onto the single-stranded TG 1-3 primer in a processive manner, but could not translocate to synthesize more than one telomeric repeat. The purified telomerase core complex exhibited different activities when primers were paired with Tlc1 template at different positions. The procedure of reconstitution and purification of telomerase core enzyme we developed now allows for further mechanistic study on the functions of other subunits of telomerase holoenzyme as well as other telomerase regulation proteins.

Status: Published Type: Journal Article PubMed ID: 15813705

Topics addressed in this paper

Number of different genes curated to this paper: 4

  • To find other papers on a gene and topic, click on the colored ball in the appropriate box.
  • displays other papers with information about that topic for that gene.
  • displays other papers in SGD that are associated with that topic.
    The topic is addressed in these papers but does not describe a specific gene or chromosomal feature.
  • To go to the Locus page for a gene, click on the gene name.
Topics Genes linked to topics
EST1 EST2 EST3 TLC1
Additional Literature blue ball blue ball
Cellular Location blue ball blue ball
Function/Process blue ball blue ball
Genetic Interactions blue ball blue ball
Mutants/Phenotypes blue ball blue ball
Primary Literature blue ball blue ball
Protein Physical Properties blue ball
Protein-Nucleic Acid Interactions blue ball blue ball
Protein-protein Interactions blue ball blue ball blue ball
Strains/Constructs blue ball blue ball
Substrates/Ligands/Cofactors blue ball blue ball
Techniques and Reagents blue ball blue ball

Author Searches

To find contact information or other publications by the authors of this paper, follow these three steps:
  1. (1) Choose an author,
  2. (2) Choose a search parameter,
  3. (3) Click to implement
Page Contents: