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Felice MR, et al.  (2005) Post-transcriptional regulation of the yeast high affinity iron transport system. J Biol Chem 280(23):22181-90

Abstract: Saccharomyces cerevisiae transcriptionally regulates the expression of the plasma membrane high affinity iron transport system in response to iron need. This transport system is comprised of the products of the FET3 and FTR1 genes. We show that Fet3p and Ftr1p are post-translationally regulated by iron. Incubation of cells in high iron leads to the internalization and degradation of both Fet3p and Ftr1p. Yeast strains defective in endocytosis (end4) show a reduced iron-induced loss of Fet3p/Ftr1p. In cells with a deletion in the vacuolar protease PEP4, high iron medium leads to the accumulation of Fet3p and Ftr1p in the vacuole. Iron-induced degradation of Fet3p/Ftr1p is significantly reduced in strains containing a deletion of a gene, VTA1, which is involved in multivesicular body (MVB) sorting in yeast. Sorting through the MVB can involve ubiquitination. We demonstrate that Ftr1p is ubiquitinated while Fet3p is not ubiquitinated. Iron-induced internalization and degradation of Fet3p/Ftr1p occurs in a mutant strain of the E3 ubiquitin ligase RSP5 (rsp5-1), suggesting that Rsp5p is not required. Internalization of Fet3p/Ftr1p is specific for iron and requires both an active Fet3p and Ftr1p, indicating that it is the transport of iron through the iron permease Ftr1p that is responsible for the internalization and degradation of the Fet3p/Ftr1p complex.

Status: Published

Type: Journal Article

PubMed ID: 15817488

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Felice MR De Domenico I Li L Ward DM Bartok B Musci G Kaplan J Papers in SGD Colleagues in SGD PubMed Google Scholar

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