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Zhu H, et al.  (2000) Analysis of yeast protein kinases using protein chips. Nat Genet 26(3):283-9

Abstract: We have developed a novel protein chip technology that allows the high-throughput analysis of biochemical activities, and used this approach to analyse nearly all of the protein kinases from Saccharomyces cerevisiae. Protein chips are disposable arrays of microwells in silicone elastomer sheets placed on top of microscope slides. The high density and small size of the wells allows for high-throughput batch processing and simultaneous analysis of many individual samples. Only small amounts of protein are required. Of 122 known and predicted yeast protein kinases, 119 were overexpressed and analysed using 17 different substrates and protein chips. We found many novel activities and that a large number of protein kinases are capable of phosphorylating tyrosine. The tyrosine phosphorylating enzymes often share common amino acid residues that lie near the catalytic region. Thus, our study identified a number of novel features of protein kinases and demonstrates that protein chip technology is useful for high-throughput screening of protein biochemical activity.

Status: Published Type: Journal Article PubMed ID: 11062466

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Number of different genes curated to this paper: 123

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Topics Topics not linked to Genes Genes linked to topics (#1 - 10 )
AKL1 ARK1 ATG1 AXL2 BCK1 BUB1 BUD32 CAK1 CBK1 CDC15
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Function/Process blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Large-scale protein detection yg ball
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Omics yg ball
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Topics Genes linked to topics (#11 - 20 )
CDC28 CDC5 CDC7 CHK1 CKA1 CLA4 CMK1 CMK2 CTK1 CTK2
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Topics Genes linked to topics (#21 - 30 )
CTK3 DBF2 DBF20 DBF4 DUN1 ELM1 ENV7 FMP48 FPK1 FRK1
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Function/Process blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#31 - 40 )
FUS3 GIC2 GIN4 HAL5 HOG1 HRK1 HRR25 HSL1 IME2 IPL1
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Topics Genes linked to topics (#41 - 50 )
IRE1 KCC4 KDX1 KIC1 KIN1 KIN2 KIN28 KIN3 KIN4 KIN82
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Function/Process blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#51 - 60 )
KKQ8 KNS1 KSP1 KSS1 MCK1 MEC1 MEK1 MKK1 MKK2 MPS1
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Function/Process blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Regulation of blue ball
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Topics Genes linked to topics (#61 - 70 )
MRK1 NNK1 NPR1 PBS2 PHO85 PKC1 PKH1 PKH2 PKH3 PKP1
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Function/Process blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#71 - 80 )
PKP2 PRK1 PRR1 PRR2 PRS5 PSK1 PSK2 PTK1 PTK2 RAD53
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball
Function/Process blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Mutants/Phenotypes blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Primary Literature blue ball blue ball blue ball blue ball
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Regulation of blue ball
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Topics Genes linked to topics (#81 - 90 )
RAD9 RCK1 RCK2 RED1 RIM11 RTK1 SAK1 SAT4 SCH9 SGV1
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Function/Process blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Mutants/Phenotypes blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein Processing/Modification/Regulation blue ball blue ball
Regulation of blue ball blue ball
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Topics Genes linked to topics (#91 - 100 )
SKM1 SKS1 SKY1 SLN1 SLT2 SMK1 SNF1 SPS1 SSK2 SSK22
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Topics Genes linked to topics (#101 - 110 )
SSN3 STE11 STE20 STE7 SWE1 SWI6 TDA1 TOS3 TPK1 TPK2
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Function/Process blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Mutants/Phenotypes blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein Processing/Modification/Regulation blue ball
Regulation of blue ball
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Topics Genes linked to topics (#111 - 120 )
TPK3 TUB4 TWF1 VHS1 YAK1 YCK1 YCK2 YCK3 YGK3 YPK1
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Function/Process blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Mutants/Phenotypes blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Primary Literature blue ball
Protein Processing/Modification/Regulation blue ball
Regulation of blue ball
Strains/Constructs blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Substrates/Ligands/Cofactors blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Techniques and Reagents blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball

Topics Genes linked to topics (#121 - 123 )
YPK2 YPK3 YPL150W
Additional Literature blue ball
Function/Process blue ball blue ball blue ball
Mutants/Phenotypes blue ball blue ball blue ball
Primary Literature blue ball blue ball
Strains/Constructs blue ball blue ball blue ball
Substrates/Ligands/Cofactors blue ball blue ball blue ball
Techniques and Reagents blue ball blue ball blue ball

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