Boa S, et al. (2003) Saccharomyces cerevisiae Set1p is a methyltransferase specific for lysine 4 of histone H3 and is required for efficient gene expression. Yeast 20(9):827-35
Abstract: Several homologues of the Drosophila Su(var)3-9 protein were recently reported to methylate lysine 9 of histone H3. Whereas this methylation signal served to recruit heterochromatin-associated proteins to transcriptionally silenced regions, histone H3 methylated at lysine 4 was associated with transcriptionally active areas of the genome. These findings suggested that the interplay between lysine 4 and 9 methylation is crucial in eukaryotic gene regulation. Here we provide evidence that Saccharomyces cerevisiae Set1p is a methyltransferase specific for lysine 4 of histone H3. In addition, we show that the absence of Set1p and lysine 4 methylation result in decreased transcription of approximately 80% of the genes in S. cerevisiae. Hierarchical clustering analysis of the set1(-) expression profile revealed a correspondence to that of a mad2(-) strain, suggesting that the transcriptional defect in the set1(-) strain may be due to changes in chromatin structure. These findings establish a central role for methylation of histone H3 lysine 4 in transcriptional regulation.
|Status: Published||Type: Journal Article | Research Support, Non-U.S. Gov't||PubMed ID: 12845608|
Topics addressed in this paper
Number of different genes curated to this paper: 4
- To go to the Locus page for a gene, click on the gene name.
|Topics||Topics not linked to Genes||Genes|
|Genomic expression study|
|Non-Fungal Related Genes/Proteins|
|Protein Sequence Features|
|Protein/Nucleic Acid Structure|