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Goehring AS, et al.  (2003) Synthetic lethal analysis implicates Ste20p, a p21-activated potein kinase, in polarisome activation. Mol Biol Cell 14(4):1501-16

Abstract: The p21-activated kinases Ste20p and Cla4p carry out undefined functions that are essential for viability during budding in Saccharomyces cerevisiae. To gain insight into the roles of Ste20p, we have used a synthetic lethal mutant screen to identify additional genes that are required in the absence of Cla4p. Altogether, we identified 65 genes, including genes with roles in cell polarity, mitosis, and cell wall maintenance. Herein, we focus on a set that defines a function carried out by Bni1p and several of its interacting proteins. We found that Bni1p and a group of proteins that complex with Bni1p (Bud6p, Spa2p, and Pea2p) are essential in a cla4delta mutant background. Bni1p, Bud6p, Spa2, and Pea2p are members of a group of polarity determining proteins referred to as the polarisome. Loss of polarisome proteins from a cla4delta strain causes cells to form elongated buds that have mislocalized septin rings. In contrast, other proteins that interact with or functionally associate with Bni1p and have roles in nuclear migration and cytokinesis, including Num1p and Hof1p, are not essential in the absence of Cla4p. Finally, we have found that Bni1p is phosphorylated in vivo, and a substantial portion of this phosphorylation is dependent on STE20. Together, these results suggest that one function of Ste20p may be to activate the polarisome complex by phosphorylation of Bni1p.

Status: Published Type: Journal Article PubMed ID: 12686605

Topics addressed in this paper

Number of different genes curated to this paper: 69

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Topics Topics not linked to Genes Genes linked to topics (#1 - 10 )
ACT1 APC9 ASF1 BCK1 BEM1 BEM2 BEM4 BNI1 BUD6 CDC3
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Cellular Location blue ball blue ball
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Topics Genes linked to topics (#11 - 20 )
CHS3 CHS5 CHS6 CHS7 CLA4 CTF18 CTF4 CTF8 DCC1 DYN2
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Topics Genes linked to topics (#21 - 30 )
EDE1 ELP2 ELP6 FAB1 FKH2 GIM3 GIM5 HOF1 HOS4 ITC1
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Topics Genes linked to topics (#31 - 40 )
IXR1 MGA2 NCS2 NIP100 NUM1 PEA2 PRE9 RAD54 RIM20 RPL19A
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Topics Genes linked to topics (#41 - 50 )
RPL23A RPL24A RPL35A RPN10 RPS21B RTT101 RTT106 RXT2 SAP30 SEM1
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Topics Genes linked to topics (#51 - 60 )
SGF11 SGF73 SHS1 SKT5 SLK19 SLT2 SMI1 SPA2 SPO12 STE20
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Topics Genes linked to topics (#61 - 69 )
SWD3 SWI4 UBA4 UBP8 URE2 VAC14 YBL062W YBR174C YML094C-A
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