SGD Paper Help


Page Contents:
Valerius O, et al.  (2002) Multiple factors prevent transcriptional interference at the yeast ARO4-HIS7 locus. J Biol Chem 277(24):21440-5

Abstract: Increased transcriptional activity may cause transcriptional interference in organisms with compact genomes such as the yeast Saccharomyces cerevisiae. Replacement of the yeast ARO4 promoter by the stronger ACT1 promoter increases ARO4 transcription and simultaneously reduces the basal transcription of the downstream HIS7 gene. The open reading frames of ARO4 and HIS7 are tandemly transcribed and are separated by 416 bp. In wild-type cells, a nuclease-resistant site suggests that the two genes are separated by a single positioned nucleosome. Transcriptional interference correlates with Micrococcus nuclease accessibility of this otherwise nuclease-resistant site. Deletion analyses of the region between the two open reading frames revealed that transcriptional interference increases upon removal of either parts of the ARO4 3' end or HIS7 promoter sequences. The abolishment of the Abf1p-binding site within the HIS7 promoter significantly enhances transcriptional interference, resulting in a histidine auxotrophic strain. Our data suggest that the yeast cell prevents transcriptional interference by the combined action of efficient ARO4 transcription termination, the positioning of a fixed nucleosome, and transcription factor binding to the HIS7 promoter.

Status: Published Type: Journal Article PubMed ID: 11937506

Topics addressed in this paper

Number of different genes curated to this paper: 4

  • To find other papers on a gene and topic, click on the colored ball in the appropriate box.
  • displays other papers with information about that topic for that gene.
  • displays other papers in SGD that are associated with that topic.
    The topic is addressed in these papers but does not describe a specific gene or chromosomal feature.
  • To go to the Locus page for a gene, click on the gene name.
Topics Genes linked to topics
ABF1 ACT1 ARO4 HIS7
Additional Literature blue ball
DNA/RNA Sequence Features blue ball blue ball
Function/Process blue ball blue ball
Genetic Interactions blue ball
Mapping blue ball
Mutants/Phenotypes blue ball blue ball
Primary Literature blue ball blue ball blue ball
Protein-Nucleic Acid Interactions blue ball blue ball
Strains/Constructs blue ball blue ball
Substrates/Ligands/Cofactors blue ball
Techniques and Reagents blue ball
Transcription blue ball blue ball

Author Searches

To find contact information or other publications by the authors of this paper, follow these three steps:
  1. (1) Choose an author,
  2. (2) Choose a search parameter,
  3. (3) Click to implement
Page Contents: