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Parrish W, et al.  (2002) The cytoplasmic end of transmembrane domain 3 regulates the activity of the Saccharomyces cerevisiae G-protein-coupled alpha-factor receptor. Genetics 160(2):429-43

Abstract: The binding of [alpha]-factor to its receptor (Ste2p) activates a G-protein-signaling pathway leading to conjugation of MATa cells of the budding yeast S. cerevisiae. We conducted a genetic screen to identify constitutively activating mutations in the N-terminal region of the [alpha]-factor receptor that includes transmembrane domains 1--5. This approach identified 12 unique constitutively activating mutations, the strongest of which affected polar residues at the cytoplasmic ends of transmembrane domains 2 and 3 (Asn(84) and Gln(149), respectively) that are conserved in the [alpha]-factor receptors of divergent yeast species. Targeted mutagenesis, in combination with molecular modeling studies, suggested that Gln(149) is oriented toward the core of the transmembrane helix bundle where it may be involved in mediating an interaction with Asn(84). These residues appear to play specific roles in maintaining the inactive conformation of the protein since a variety of mutations at either position cause constitutive receptor signaling. Interestingly, the activity of many mammalian G-protein-coupled receptors is also regulated by conserved polar residues (the E/DRY motif) at the cytoplasmic end of transmembrane domain 3. Altogether, the results of this study suggest a conserved role for the cytoplasmic end of transmembrane domain 3 in regulating the activity of divergent G-protein-coupled receptors.

Status: Published

Type: Journal Article

PubMed ID: 11861550

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Parrish W Eilers M Ying W Konopka JB Papers in SGD Colleagues in SGD PubMed Google Scholar

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