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Bushnell DA, et al. (2001) Selenomethionine incorporation in Saccharomyces cerevisiae RNA polymerase II. Structure 9(1):R11-4
Abstract: A protocol for the incorporation of SeMet into yeast proteins is described. Incorporation at a level of about 50% suffices for the location of Se sites in an anomalous difference Fourier map of the 0.5 MDa yeast RNA polymerase II. This shows the utility of the approach as an aid in the model-building of large protein complexes.
| Status: Published | Type: Journal Article | PubMed ID: 11342141 |
Topics addressed in this paper
Number of different genes curated to this paper: 12
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| Topics | Genes linked to topics (#1 - 10 ) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| RPB10 | RPB11 | RPB2 | RPB3 | RPB4 | RPB5 | RPB7 | RPB8 | RPB9 | RPC10 | |
| Additional Literature | | | | | | | | | | |
| Protein/Nucleic Acid Structure | | | | | | | | | | |
| Techniques and Reagents | | | | | | | | | | |
| Topics | Genes linked to topics (#11 - 12 ) | |
|---|---|---|
| RPO21 | RPO26 | |
| Additional Literature | | |
| Protein/Nucleic Acid Structure | | |
| Techniques and Reagents | | |
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