Brow DA and Geiduschek EP (1987) Modulation of yeast 5 S rRNA synthesis in vitro by ribosomal protein YL3. A possible regulatory loop. J Biol Chem 262(29):13953-8
Abstract: Synthesis of yeast 5 S rRNA in a homologous cell-free system has previously been shown to be inhibited by exogenous yeast 5 S rRNA. This inhibition is diminished when 5 S rDNA is first incubated with the cell-free system, implying the presence of a transcription factor IIIA analog in yeast, and is dependent on the nucleotide sequence of the 5 S rRNA added. A two-dimensional gel electrophoresis method was used to demonstrate assembly of newly synthesized 5 S rRNA into a ribonucleoprotein complex containing ribosomal protein YL3. Immunodepletion of the cell-free system with anti-YL3 IgG was found to increase the sensitivity of 5 S rRNA synthesis to inhibition by 5 S rRNA. We propose a model for the regulation of yeast 5 S rRNA synthesis in which YL3 competes with the presumptive transcription factor IIIA analog for binding of 5 S rRNA.
|Status: Published||Type: Journal Article||PubMed ID: 3308884|
Topics addressed in this paper
Number of different genes curated to this paper: 8
- To go to the Locus page for a gene, click on the gene name.