SGD Paper 
Kim HC, et al. (2001) Cloning, expression, and complementation test of the RNA lariat debranching enzyme cDNA from mouse. Mol Cells 11(2):198-203
Abstract: The RNA lariat debranching enzyme of mouse (mDBR1) was cloned by screening a NIH/3T3 cDNA library. The sequence of full-length mDBR1 cDNA containeda single 515 amino acid open reading frame of 58 kDa protein. Comparison of the amino acid sequence of mDBR1 to other DBR proteins showed 40%, 44%, 43%, 42%, and 80% identity to Saccharomyces cerevisiae, Schizosaccharomyces pombe, Caenorhabditis elegans, Drosophila melanogaster, and human debranching enzymes, respectively. The mDBR1 cDNA was shown to be functional in an interspecies specific complementation experiment, and an in vitro debranching enzyme assay. Mouse DBR1 could complement the intron accumulation phenotype of a S. cerevisiae dbrl null mutant strain. However, the level of complementation depended on the copy number of the mDBR1 cDNA. The integration of the mDBR1 cDNA in the chromosome of S. pombe also complemented both intron accumulation and slow growth phenotypes of the S. pombe dbr1 knock-out mutant strain.
| Status: Published | Type: Journal Article | PubMed ID: 11355701 |
Topics addressed in this paper
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| Topics | Genes linked to topics |
|---|---|
| DBR1 | |
| Cross-species Expression |  |
| Fungal Related Genes/Proteins | |
| Mutants/Phenotypes | |
| Non-Fungal Related Genes/Proteins | |
| Primary Literature | |
| Strains/Constructs | |
