SGD Paper 
Roy S, et al. (1982) Procedure for C2 deuteration of nucleic acids and determination of A psi 31 pseudouridine conformation by nuclear Overhauser effect in yeast tRNAPhe. Nucleic Acids Res 10(24):8341-9
Abstract: Nuclear Overhauser effect (NOE) combined with semispecific deuteration provides a general strategy for identification of exchangeable protons in nucleic base pairs, and has been extended to NOEs involving purine C2 protons in tRNA. Deuterated tri-ethyl orthoformate was condensed with 5(4)-amino imidazole 4(5)-carboxamide to yield C2 deuterated hypoxanthine. C2 deuterated hypoxanthine was fed to a purine requiring mutant of yeast and C2 deuterated yeast tRNAPhe was isolated. This C2 deuterated tRNAPhe was used to identify A psi 31 and U8-A14. A psi 31 was found to be bonded through N1H. The utility of C2 deuteration in nucleic acid NMR is thus demonstrated.
| Status: Published | Type: Journal Article | PubMed ID: 6761652 |
Topics addressed in this paper
Number of different genes curated to this paper: 11
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| Topics | Genes linked to topics (#1 - 10 ) | |||||||||
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| tF(GAA)B | tF(GAA)D | tF(GAA)F | tF(GAA)G | tF(GAA)H1 | tF(GAA)H2 | tF(GAA)M | tF(GAA)N | tF(GAA)P1 | tF(GAA)P2 | |
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| Techniques and Reagents | | | | | | | | | | |
| Topics | Genes linked to topics (#11 ) |
|---|---|
| tF(GAA)Q | |
| Additional Literature | |
| DNA/RNA Sequence Features | |
| Techniques and Reagents | |
