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Lussier M, et al.  (1997) Large scale identification of genes involved in cell surface biosynthesis and architecture in Saccharomyces cerevisiae. Genetics 147(2):435-50

Abstract: The sequenced yeast genome offers a unique resource for the analysis of eukaryotic cell function and enables genome-wide screens for genes involved in cellular processes. We have identified genes involved in cell surface assembly by screening transposon-mutagenized cells for altered sensitivity to calcofluor white, followed by supplementary screens to further characterize mutant phenotypes. The mutated genes were directly retrieved from genomic DNA and then matched uniquely to a gene in the yeast genome database. Eighty-two genes with apparent perturbation of the cell surface were identified, with mutations in 65 of them displaying at least one further cell surface phenotype in addition to their modified sensitivity to calcofluor. Fifty of these genes were previously known, 17 encoded proteins whose function could be anticipated through sequence homology or previously recognized phenotypes and 15 genes had no previously known phenotype.

Status: Published Type: Journal Article PubMed ID: 9335584

Topics addressed in this paper

Number of different genes curated to this paper: 72

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Topics Genes linked to topics (#1 - 10 )
ACS1 ALG12 ALG9 ARG7 ATG32 BUD8 COX11 CWP2 DCG1 DFG16
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Topics Genes linked to topics (#11 - 20 )
DIT2 ECM10 ECM11 ECM12 ECM13 ECM14 ECM15 ECM16 ECM18 ECM19
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Topics Genes linked to topics (#21 - 30 )
ECM2 ECM21 ECM23 ECM25 ECM27 ECM29 ECM30 ECM31 ECM32 ECM33
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Topics Genes linked to topics (#31 - 40 )
ECM38 ECM4 ECM5 ECM7 ECM8 ECM9 FOX2 GSF2 HAL5 HCA4
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Topics Genes linked to topics (#41 - 50 )
IFM1 IMP2' KRE2 KRE6 KTR6 LAG2 MEC3 MET5 MKS1 MRE11
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Topics Genes linked to topics (#51 - 60 )
MSN1 PAH1 PEX6 PGS1 RGT2 RNH1 ROM2 RPC34 SKI6 SLG1
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Topics Genes linked to topics (#61 - 70 )
SLN1 SNF3 SPE1 SPE3 SSK2 STF2 TFC1 URA7 VAN1 YCK2
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Topics Genes linked to topics (#71 - 72 )
YCL007C YEF3
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