Ozkaynak E, et al. (1987) The yeast ubiquitin genes: a family of natural gene fusions. EMBO J 6(5):1429-39
Abstract: Ubiquitin is a 76-residue protein highly conserved among eukaryotes. Conjugation of ubiquitin to intracellular proteins mediates their selective degradation in vivo. We describe a family of four ubiquitin-coding loci in the yeast Saccharomyces cerevisiae. UBI1, UBI2 and UBI3 encode hybrid proteins in which ubiquitin is fused to unrelated ('tail') amino acid sequences. The ubiquitin coding elements of UBI1 and UBI2 are interrupted at identical positions by non-homologous introns. UBI1 and UBI2 encode identical 52-residue tails, whereas UBI3 encodes a different 76-residue tail. The tail amino acid sequences are highly conserved between yeast and mammals. Each tail contains a putative metal-binding, nucleic acid-binding domain of the form Cys-X2-4-Cys-X2-15-Cys-X2-4-Cys, suggesting that these proteins may function by binding to DNA. The fourth gene, UBI4, encodes a polyubiquitin precursor protein containing five ubiquitin repeats in a head-to-tail, spacerless arrangement. All four ubiquitin genes are expressed in exponentially growing cells, while in stationary-phase cells the expression of UBI1 and UBI2 is repressed. The UBI4 gene, which is strongly inducible by starvation, high temperatures and other stresses, contains in its upstream region strong homologies to the consensus 'heat shock box' nucleotide sequence. Elsewhere we show that the essential function of the UBI4 gene is to provide ubiquitin to cells under stress.
|Status: Published||Type: Journal Article||PubMed ID: 3038523|
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