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Xiang RH and Lee JC  (1989) Identification of proteins crosslinked to RNA in 40S ribosomal subunits of Saccharomyces cerevisiae. Biochimie 71(11-12):1201-4

Abstract: RNA-protein crosslinks were introduced into the 40S ribosomal subunits from Saccharomyces cerevisiae by mild UV treatment. Proteins crosslinked to the 18S rRNA molecule were separated from free proteins by repeated extraction of the treated subunits and centrifugation in glycerol gradients. After digestion with RNase to remove the RNA molecules, proteins were radio-labeled with 125I and identified by electrophoresis on two-dimensional polyacrylamide gels with carrier total 40S ribosomal proteins and autoradiography. Proteins S2, S7, S13, S14, S17/22/27, and S18 were linked to the 18S rRNA. A shorter period of irradiation resulted in crosslinking of S2 and S17/22/27 only. Several of these proteins were previously demonstrated to be present in ribosomal core particles or early assembled proteins.

Status: Published Type: Journal Article PubMed ID: 2517480

Topics addressed in this paper

Number of different genes curated to this paper: 15

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Topics Genes linked to topics (#1 - 10 )
RPS11A RPS11B RPS13 RPS17A RPS17B RPS2 RPS27A RPS27B RPS4A RPS4B
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Alias blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Protein Physical Properties blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein-Nucleic Acid Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein-protein Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball

Topics Genes linked to topics (#11 - 15 )
RPS5 RPS8A RPS8B RPS9A RPS9B
Additional Literature blue ball blue ball blue ball blue ball blue ball
Alias blue ball blue ball blue ball blue ball blue ball
Protein Physical Properties blue ball blue ball blue ball blue ball blue ball
Protein-Nucleic Acid Interactions blue ball blue ball blue ball blue ball blue ball
Protein-protein Interactions blue ball blue ball blue ball blue ball blue ball

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