SGD Paper 
Boonchird C, et al. (1991) Determination of amino acid sequences involved in the processing of the ARG5/ARG6 precursor in Saccharomyces cerevisiae. Eur J Biochem 199(2):325-35
Abstract: In Saccharomyces cerevisiae, the ARG5/ARG6 locus encodes both acetylglutamate kinase and acetylglutamyl-phosphate reductase, localized in the mitochondria. Genetic analysis, determination of the nucleotide sequence of the ARG5/ARG6 gene and identification of the transcript indicate that it encodes a single translation product containing two enzyme activities. However, analysis of cellular extracts revealed that the activities are completely separable. In this work, we define different domains in the ARG5/ARG6 polypeptide; a mitochondrial target sequence and the acetylglutamate-kinase and acetylglutamyl-phosphate-reductase domains. We show that deletions in the N-terminal end of the protein and point mutations in the junction region between the acetylglutamate-kinase and acetylglutamyl-phosphate-reductase domains lead to the accumulation of large precursor. Our data support the idea that import of the ARG5/ARG6 precursor into the mitochondria is required for its processing into two mature enzymes.
| Status: Published | Type: Journal Article | PubMed ID: 1649049 |
Topics addressed in this paper
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| Topics | Genes linked to topics |
|---|---|
| ARG5,6 | |
| Cellular Location |  |
| Function/Process | |
| Mutants/Phenotypes | |
| Primary Literature | |
| Protein Processing/Modification/Regulation | |
| Protein Sequence Features | |
| RNA Levels and Processing | |
| Strains/Constructs | |
