SGD Paper 
Serre MC and Jayaram M (1992) Half-site strand transfer by step-arrest mutants of yeast site-specific recombinase Flp. J Mol Biol 225(3):643-9
Abstract: The Flp recombinase of Saccharomyces cerevisae can mediate strand transfer within a half-site, between two half-sites and between a half-site and a full-site. The ability of "step-arrest" mutants of Flp to partake in half-site reactions has been examined. Arg308 variants of Flp, which show little or no strand cleavage in reactions with normal full-sites, execute significant levels of strand transfer in half-site reactions. On the other hand, His305 variants of Flp, which normally accumulate the strand cleavage product from full-sites but do not complete strand transfer, yield only minute amounts of strand transfer products from half-sites. As would be predicted, the step-arrest mutants are unable to produce "normal" or "reverse" recombinants between a half-site and a full-site. The Flp protein is able to form higher-order complexes in association with a half-site. The step-arrest mutants of Flp show specific defects in forming these complexes.
| Status: Published | Type: Journal Article | PubMed ID: 1602475 |
Topics addressed in this paper
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| Topics | Genes linked to topics |
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| FLP1 | |
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| Protein Sequence Features | |
| Protein-Nucleic Acid Interactions | |
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