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Entian KD, et al.  (1999) Functional analysis of 150 deletion mutants in Saccharomyces cerevisiae by a systematic approach. Mol Gen Genet 262(4-5):683-702

Abstract: In a systematic approach to the study of Saccharomyces cerevisiae genes of unknown function, 150 deletion mutants were constructed (1 double, 149 single mutants) and phenotypically analysed. Twenty percent of all genes examined were essential. The viable deletion mutants were subjected to 20 different test systems, ranging from high throughput to highly specific test systems. Phenotypes were obtained for two-thirds of the mutants tested. During the course of this investigation, mutants for 26 of the genes were described by others. For 18 of these the reported data were in accordance with our results. Surprisingly, for seven genes, additional, unexpected phenotypes were found in our tests. This suggests that the type of analysis presented here provides a more complete description of gene function.

Status: Published Type: Journal Article PubMed ID: 10628851

Topics addressed in this paper

Number of different genes curated to this paper: 140

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Topics Genes linked to topics (#1 - 10 )
ABM1 AIM3 ALT1 AMN1 APD1 APL2 ATG36 AVT3 BIO3 BIO4
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Topics Genes linked to topics (#11 - 20 )
BIO5 BNA3 BUD4 CFD1 CHA4 CMC1 CNS1 CSF1 DJP1 DSL1
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Topics Genes linked to topics (#21 - 30 )
DSN1 ECM27 ECM31 ERG25 EXO5 EXO84 FES1 FOL1 GIS2 GIS3
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Topics Genes linked to topics (#31 - 40 )
GPM3 GPX2 GRS1 GWT1 HIR3 HOM6 ICS2 ICS3 ICT1 IFA38
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Alias blue ball
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Topics Genes linked to topics (#41 - 50 )
ILM1 IML1 IML2 IML3 IOC2 IST1 IST2 IST3 ISW1 KAP104
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Topics Genes linked to topics (#51 - 60 )
KHA1 LST7 LTO1 MAM3 MGM101 MMS4 MPA43 MPH1 MRPL17 MRPL49
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Function/Process blue ball
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Topics Genes linked to topics (#61 - 70 )
MSL1 MTO1 MYO3 NET1 NMD5 NNF1 NPL4 NRD1 NYV1 OCT1
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Mutants/Phenotypes blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#71 - 80 )
PBY1 PCA1 PDR17 PEX32 PHO88 PHO89 PHS1 POL30 POP7 PRS5
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Topics Genes linked to topics (#81 - 90 )
PRY1 PRY3 PSH1 PTC4 RAD16 RFC5 RMA1 RPC25 RRN3 RSC1
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Mutants/Phenotypes blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Strains/Constructs blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#91 - 100 )
RSF2 RSM26 RTC2 RTC4 RXT2 SAP185 SAT4 SEC66 SGM1 SGN1
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Mutants/Phenotypes blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#101 - 110 )
SHE2 SLI15 SMC3 SNX4 SPP381 SQT1 SSE2 STE24 STR2 SUL1
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Mutants/Phenotypes blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#111 - 120 )
SUL2 TAM41 TAX4 TBS1 TEX1 TIM12 TOK1 UBS1 UFD1 VID24
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Mutants/Phenotypes blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Strains/Constructs blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball

Topics Genes linked to topics (#121 - 130 )
VPS15 VPS25 VPS70 YBR053C YBR063C YBR096W YBR242W YGR045C YGR054W YIF1
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Mutants/Phenotypes blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Primary Literature blue ball
Strains/Constructs blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball

Topics Genes linked to topics (#131 - 140 )
YJL043W YJR107W YJR111C YJR115W YJR120W YJR129C YJR141W YKL147C YOL057W YSA1
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Mutants/Phenotypes blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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