Casal M, et al. (1995) Lack of lactate-proton symport activity in pck1 mutants of Saccharomyces cerevisiae. FEMS Microbiol Lett 128(3):279-82
Abstract: Mutants of Saccharomyces cerevisiae without phosphoenolpyruvate carboxykinase activity showed no measurable lactate proton symport, while mutants without fructose-1,6-bisphosphatase had normal transport activity. Incubation of a pck1 mutant, under derepression conditions in the presence of glycerol, restored the activity of the lactate-proton symport, with identical kinetic characteristics to that in the wild-type. For efficient lactate-proton symport activity, not only is an external inducer such as lactic acid needed, but also a molecule derived from the acid metabolism may be necessary.
|Status: Published||Type: Journal Article||PubMed ID: 7781975|
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