Loo S, et al. (1995) The origin recognition complex in silencing, cell cycle progression, and DNA replication. Mol Biol Cell 6(6):741-56
Abstract: This report describes the isolation of ORC5, the gene encoding the fifth largest subunit of the origin recognition complex, and the properties of mutants with a defective allele of ORC5. The orc5-1 mutation caused temperature-sensitive growth and, at the restrictive temperature, caused cell cycle arrest. At the permissive temperature, the orc5-1 mutation caused an elevated plasmid loss rate that could be suppressed by additional tandem origins of DNA replication. The sequence of ORC5 revealed a potential ATP binding site, making Orc5p a candidate for a subunit that mediates the ATP-dependent binding of ORC to origins. Genetic interactions among orc2-1 and orc5-1 and other cell cycle genes provided further evidence for a role for the origin recognition complex (ORC) in DNA replication. The silencing defect caused by orc5-1 strengthened previous connections between ORC and silencing, and combined with the phenotypes caused by orc2 mutations, suggested that the complex itself functions in both processes.
|Status: Published||Type: Journal Article||PubMed ID: 7579692|
Topics addressed in this paper
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|Topics||Genes linked to topics|
|Cell Cycle Phase Involved|
|Fungal Related Genes/Proteins|
|Protein Sequence Features|
|Protein-Nucleic Acid Interactions|