SGD Paper Help


Page Contents:
McMullin TW and Fox TD  (1993) COX3 mRNA-specific translational activator proteins are associated with the inner mitochondrial membrane in Saccharomyces cerevisiae. J Biol Chem 268(16):11737-41

Abstract: Three yeast proteins coded by nuclear genes, PET54, PET122, and PET494, are specifically required to activate translation of the mitochondrially coded COX3 mRNA, and one of them, PET122, has been shown to interact functionally with both the mitochondrial ribosomal small subunit and the COX3 mRNA leader. To determine the location of these proteins within mitochondria, we have used antisera directed against them to assay for their presence in submitochondrial fractions. Approximately half of the PET54 protein present in mitochondria from wild-type cells pelleted with membranes, while half was released from disrupted mitochondria in soluble form. The membrane-bound PET54 floated with the inner membrane during buoyant density gradient centrifugation, but was efficiently solubilized by extraction with alkaline carbonate. The PET122 and PET494 proteins could only be detected in cells overproducing these proteins. PET122 was completely membrane-bound and could not be extracted with alkaline carbonate. Most of the PET494 protein pelleted with membranes and very little could be solubilized by alkaline carbonate. Both PET122 and PET494 floated with membranes during buoyant density gradient centrifugation. These data suggest the possibility that synthesis of the highly hydrophobic subunit III of cytochrome c oxidase is activated by PET54, PET122, and PET494 at the inner mitochondrial membrane. Amino-terminal sequencing of proteins isolated from mitochondria revealed that PET54 is not processed during import to mitochondria while the PET122 precursor is cleaved between the 8th and 9th residues.

Status: Published Type: Journal Article PubMed ID: 8389363

Topics addressed in this paper

Number of different genes curated to this paper: 4

  • To find other papers on a gene and topic, click on the colored ball in the appropriate box.
  • displays other papers with information about that topic for that gene.
  • displays other papers in SGD that are associated with that topic.
    The topic is addressed in these papers but does not describe a specific gene or chromosomal feature.
  • To go to the Locus page for a gene, click on the gene name.
Topics Genes linked to topics
COX3 PET122 PET494 PET54
Additional Literature blue ball
Cellular Location blue ball blue ball blue ball
Fungal Related Genes/Proteins blue ball blue ball
Primary Literature blue ball blue ball blue ball
Protein Physical Properties blue ball
Protein Processing/Modification/Regulation blue ball
RNA Levels and Processing blue ball
Strains/Constructs blue ball
Techniques and Reagents blue ball
Translational Regulation blue ball

Author Searches

To find contact information or other publications by the authors of this paper, follow these three steps:
  1. (1) Choose an author,
  2. (2) Choose a search parameter,
  3. (3) Click to implement
Page Contents: