SGD Paper Help


Page Contents:
Mead J, et al.  (1996) The yeast alpha2 and Mcm1 proteins interact through a region similar to a motif found in homeodomain proteins of higher eukaryotes. Mol Cell Biol 16(5):2135-43

Abstract: Homeodomain proteins are transcriptional regulatory factors that, in general, bind DNA with relatively low sequence specificity and affinity. One mechanism homeodomain proteins use to increase their biological specificity is through interactions with other DNA-binding proteins. We have examined how the yeast (Saccharomyces cerevisiae) homeodomain protein alpha2 specifically interacts with Mcm1, a MADS box protein, to bind DNA specifically and repress transcription. A patch of predominantly hydrophobic residues within a region preceding the homeodomain of alpha2 has been identified that specifies direct interaction with Mcm1 in the absence of DNA. This hydrophobic patch is required for cooperative DNA binding with Mcm1 in vitro and for transcriptional repression in vivo. We have also found that a conserved motif, termed YPWM, frequently found in homeodomain proteins of insects and mammals, partially functions in place of the patch in alpha2 to interact with Mcm1. These findings suggest that homeodomain proteins from diverse organisms may use analogous interaction motifs to associate with other proteins to achieve high levels of DNA binding affinity and specificity.

Status: Published Type: Journal Article PubMed ID: 8628280

Topics addressed in this paper

Number of different genes curated to this paper: 4

  • To find other papers on a gene and topic, click on the colored ball in the appropriate box.
  • displays other papers with information about that topic for that gene.
  • displays other papers in SGD that are associated with that topic.
    The topic is addressed in these papers but does not describe a specific gene or chromosomal feature.
  • To go to the Locus page for a gene, click on the gene name.
Topics Genes linked to topics
HMLALPHA2 MATALPHA MATALPHA2 MCM1
Additional Literature blue ball
Function/Process blue ball blue ball blue ball
Non-Fungal Related Genes/Proteins blue ball blue ball
Other Features blue ball blue ball
Primary Literature blue ball blue ball blue ball
Protein Sequence Features blue ball blue ball blue ball
Protein-Nucleic Acid Interactions blue ball blue ball blue ball
Protein-protein Interactions blue ball blue ball blue ball blue ball
Regulatory Role blue ball blue ball blue ball
Strains/Constructs blue ball blue ball
Substrates/Ligands/Cofactors blue ball

Author Searches

To find contact information or other publications by the authors of this paper, follow these three steps:
  1. (1) Choose an author,
  2. (2) Choose a search parameter,
  3. (3) Click to implement
Page Contents: