Christ F, et al. (2000) A model for the PI-SceIxDNA complex based on multiple base and phosphate backbone-specific photocross-links. J Mol Biol 300(4):867-75
Abstract: We have synthesized different oligodeoxynucleotides carrying, in single positions of the >36 bp recognition site of PI-SceI, photoreactive base analogues (5-iododeoxypyrimidines) or phosphate modifications (p-azidophenacylphosphorothioates) and used them in photocross-linking experiments with PI-SceI to probe the protein-DNA interface of the specific complex between the homing endonuclease PI-SceI and its DNA substrate. One base-specific and several backbone-specific cross-links were analyzed in detail: the cross-linking positions were identified by Edman degradation of isolated cross-linked peptidexoligodeoxynucleotide adducts and confirmed by site-directed mutagenesis. Based on these results and the crystal structure of PI-SceI, a model for the structure of the PI-SceIxDNA complex is proposed. Copyright 2000 Academic Press.
|Status: Published||Type: Journal Article||PubMed ID: 10891273|
Topics addressed in this paper
Number of different genes curated to this paper: 2
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