SGD Paper 
Steinmetz EJ and Brow DA (1996) Repression of gene expression by an exogenous sequence element acting in concert with a heterogeneous nuclear ribonucleoprotein-like protein, Nrd1, and the putative helicase Sen1. Mol Cell Biol 16(12):6993-7003
Abstract: We have fortuitously identified a nucleotide sequence that decreases expression of a reporter gene in the yeast Saccharomyces cerevisiae 20-fold when inserted into an intron. The primary effect of the insertion is a decrease in pre-mRNA abundance accompanied by the appearance of 3'-truncated transcripts, consistent with premature transcriptional termination and/or pre-mRNA degradation. Point mutations in the cis element relieve the negative effect, demonstrating its sequence specificity. A novel yeast protein, named Nrd1, and a previously identified putative helicase, Sen1, help mediate the negative effect of the cis element. Sen1 is an essential nuclear protein that has been implicated in a variety of nuclear functions. Nrd1 has hallmarks of a heterogeneous nuclear ribonucleoprotein, including an RNA recognition motif, a region rich in RE and RS dipeptides, and a proline- and glutamine-rich domain. An N-terminal domain of Nrd1 may facilitate direct interaction with RNA polymerase II. Disruption of the NRD1 gene is lethal, yet C-terminal truncations that delete the RNA recognition motif and abrogate the negative effect of the cis element nevertheless support cell growth. Thus, expression of a gene containing the cis element could be regulated through modulation of the activity of Nrd1. The recent identification of Nrd1-related proteins in mammalian cells suggests that this potential regulatory pathway is widespread among eukaryotes.
| Status: Published | Type: Journal Article | PubMed ID: 8943355 |
Topics addressed in this paper
Number of different genes curated to this paper: 2
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| Topics | Genes linked to topics | |
|---|---|---|
| NRD1 | SEN1 | |
| Function/Process |  | |
| Fungal Related Genes/Proteins | | |
| Genetic Interactions | | |
| Mutants/Phenotypes | | |
| Non-Fungal Related Genes/Proteins | | |
| Other Features | | |
| Primary Literature | | |
| Protein Sequence Features | | |
| Protein-Nucleic Acid Interactions | | |
| Protein-protein Interactions | | |
| Regulatory Role | | |
| Strains/Constructs | | |
