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Dubois E and Messenguy F (1991) In vitro studies of the binding of the ARGR proteins to the ARG5,6 promoter. Mol Cell Biol 11(4):2162-8
Abstract: ARGRI, ARGRII, and ARGRIII regulatory proteins control the expression of arginine anabolic and catabolic genes in Saccharomyces cerevisiae. We show here that they are also required in vitro to observe a protein-DNA complex with the promoter of the ARG5,6 gene. The specific binding of ARGR proteins in vitro is stimulated by arginine. Antibodies raised against a synthetic MCM1 polypeptide retard the migration of ARGR-DNA complex on gel mobility shift assays. This result suggests that MCM1 could be an additional regulatory element of arginine metabolism.
| Status: Published | Type: Journal Article | PubMed ID: 2005902 |
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