SGD Paper 
Washington MT, et al. (1999) Fidelity and processivity of Saccharomyces cerevisiae DNA polymerase eta. J Biol Chem 274(52):36835-8
Abstract: The yeast RAD30 gene functions in error-free replication of UV-damaged DNA, and RAD30 encodes a DNA polymerase, pol eta, that has the ability to efficiently and correctly replicate past a cis-syn-thymine-thymine dimer in template DNA. To better understand the role of pol eta in damage bypass, we examined its fidelity and processivity on nondamaged DNA templates. Steady-state kinetic analyses of deoxynucleotide incorporation indicate that pol eta has a low fidelity, misincorporating deoxynucleotides with a frequency of about 10(-2) to 10(-3). Also pol eta has a low processivity, incorporating only a few nucleotides before dissociating. We suggest that pol eta's low fidelity reflects a flexibility in its active site rendering it more tolerant of DNA damage, while its low processivity limits its activity to reduce errors.
| Status: Published | Type: Journal Article | PubMed ID: 10601233 |
Topics addressed in this paper
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| Topics | Genes linked to topics |
|---|---|
| RAD30 | |
| Function/Process |  |
| Other Features | |
| Primary Literature | |
| Techniques and Reagents | |
