Zaman Z, et al. (1992) A 3' transcriptional enhancer within the coding sequence of a yeast gene encoding the common subunit of two multi-enzyme complexes. Mol Microbiol 6(2):239-46
Abstract: A well-defined set of isogenic yeast strains has been constructed whereby each strain contains a different LPD::lacZ gene fusion integrated at the ura3 locus. These LPD::lacZ fusions differ in the amount of the LPD1 gene (encoding lipoamide dehydrogenase) that is fused to the lacZ reporter. Comparison of the beta-galactosidase activities of each strain during growth on glucose or ethanol revealed that some part of the LPD1 coding region between +13 and +700 is involved in activating gene expression ina carbon source-dependent manner. This activation occurs at the mRNA level, and is not mediated by changes in mRNA stability. Therefore, the LPD1 gene appears to contain a transcriptional enhancer that lies 3' to the transcriptional start site, and which responds to carbon source.
| Status: Published | Type: Journal Article | PubMed ID: 1545708 |
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| Topics | Genes linked to topics |
|---|---|
| LPD1 | |
| DNA/RNA Sequence Features | |
| Primary Literature | |
| Regulation of | |
| RNA Levels and Processing | |
| Strains/Constructs | |
| Transcription | |




