STE2/YFL026W Literature Guide Help

Other names published for STE2: YFL026W

STE2 - Techniques and Reagents (51)

ReferenceOther Genes Addressed
Mathew E, et al.  (2013) Functional fusions of T4 lysozyme in the third intracellular loop of a G protein-coupled receptor identified by a random screening approach in yeast. Protein Eng Des Sel 26(1):59-71
Mathew E, et al.  (2011) Differential interactions of fluorescent agonists and antagonists with the yeast G protein coupled receptor Ste2p. J Mol Biol 409(4):513-28
Stoneman M, et al.  (2011) In vivo Quantification of G Protein Coupled Receptor Interactions using Spectrally Resolved Two-photon Microscopy.LID - 10.3791/2247 [doi]LID - 2247 [pii] J Vis Exp (47)
Clark KM, et al.  (2010) Purification of transmembrane proteins from Saccharomyces cerevisiae for X-ray crystallography. Protein Expr Purif 71(2):207-23
Dosil M and Konopka JB  (2010) Strategies for isolating constitutively active and dominant-negative pheromone receptor mutants in yeast. Methods Enzymol 485():329-48
Iguchi Y, et al.  (2010) Control of signalling properties of human somatostatin receptor subtype-5 by additional signal sequences on its amino-terminus in yeast. J Biochem 147(6):875-84
Dowell SJ and Brown AJ  (2009) Yeast assays for g protein-coupled receptors. Methods Mol Biol 552:213-29
Tanaka H and Yi TM  (2009) Synthetic morphology using alternative inputs. PLoS One 4(9):e6946
Umanah GK, et al.  (2009) Cross-linking of a DOPA-containing peptide ligand into its G protein-coupled receptor. Biochemistry 48(9):2033-44
Huang LY, et al.  (2008) Unnatural amino acid replacement in a yeast G protein-coupled receptor in its native environment. Biochemistry 47(20):5638-48
Mo Y, et al.  (2008) Detergent-Associated Solution Conformations of Helical and beta-Barrel Membrane Proteins. J Phys Chem B 112(42):13349-54
Moore TI, et al.  (2008) Robust spatial sensing of mating pheromone gradients by yeast cells. PLoS ONE 3(12):e3865
Lee BK, et al.  (2007) Affinity purification and characterization of a G-protein coupled receptor, Saccharomyces cerevisiae Ste2p. Protein Expr Purif 56(1):62-71
Naider F  (2007) Synthesis, biosynthesis, and characterization of transmembrane domains of a G protein-coupled receptor. Methods Mol Biol 386():95-121
Stoneman M, et al.  (2007) Protein influence on the plasma membrane dielectric properties: In vivo study utilizing dielectric spectroscopy and fluorescence microscopy. Bioelectrochemistry 70(2):542-50
Cano-Sanchez P, et al.  (2006) Effects of N- and C-terminal addition of oligolysines or native loop residues on the biophysical properties of transmembrane domain peptides from a G-protein coupled receptor. J Pept Sci 12(12):808-22
Gehret AU, et al.  (2006) Oligomerization of the yeast alpha-factor receptor: implications for dominant negative effects of mutant receptors. J Biol Chem 281(30):20698-714
Toshima JY, et al.  (2006) Spatial dynamics of receptor-mediated endocytic trafficking in budding yeast revealed by using fluorescent alpha-factor derivatives. Proc Natl Acad Sci U S A 103(15):5793-8
Naider F, et al.  (2005) Synthetic peptides as probes for conformational preferences of domains of membrane receptors. Biopolymers 80(2-3):199-213
Raicu V, et al.  (2005) Protein interaction quantified in vivo by spectrally resolved fluorescence resonance energy transfer. Biochem J 385(Pt 1):265-77
Shi C, et al.  (2005) Purification and characterization of a recombinant G-protein-coupled receptor, Saccharomyces cerevisiae Ste2p, transiently expressed in HEK293 EBNA1 cells. Biochemistry 44(48):15705-14
Son CD, et al.  (2005) Analysis of ligand-receptor cross-linked fragments by mass spectrometry. J Pept Res 65(3):418-26
Bajaj A, et al.  (2004) A fluorescent alpha-factor analogue exhibits multiple steps on binding to its G protein coupled receptor in yeast. Biochemistry 43(42):13564-78
Celic A, et al.  (2004) Intensive mutational analysis of G protein-coupled receptors in yeast. Methods Mol Biol 237():105-20
Naider F, et al.  (2004) Sexual conjugation in yeast: A paradigm to study G-protein-coupled receptor domain structure. Biopolymers 76(2):119-28
Son CD, et al.  (2004) Identification of ligand binding regions of the Saccharomyces cerevisiae alpha-factor pheromone receptor by photoaffinity cross-linking. Biochemistry 43(41):13193-203
Sugiyama K, et al.  (2004) Heterologous expression of metabotropic glutamate receptor subtype 1 in Saccharomyces cerevisiae. Appl Microbiol Biotechnol 64(4):531-6
Arevalo E, et al.  (2003) Biosynthesis and biophysical analysis of domains of a yeast G protein-coupled receptor. Biopolymers 71(4):516-31
Chang FS, et al.  (2003) A WASp homolog powers actin polymerization-dependent motility of endosomes in vivo. Curr Biol 13(6):455-63
Lin JC, et al.  (2003) Aromatic residues at the extracellular ends of transmembrane domains 5 and 6 promote ligand activation of the G protein-coupled alpha-factor receptor. Biochemistry 42(2):293-301