REP2/R0040C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 23 7.8%
Arg[R] 25 8.4%
Asn[N] 14 4.7%
Asp[D] 20 6.8%
Cys[C] 2 0.7%
Gln[Q] 10 3.4%
Glu[E] 22 7.4%
Gly[G] 11 3.7%
His[H] 1 0.3%
Ile[I] 17 5.7%
Leu[L] 28 9.5%
Lys[K] 22 7.4%
Met[M] 10 3.4%
Phe[F] 6 2.0%
Pro[P] 15 5.1%
Ser[S] 25 8.4%
Thr[T] 19 6.4%
Trp[W] 1 0.3%
Tyr[Y] 5 1.7%
Val[V] 20 6.8%
Total Length 296

Instability index
Instability index (II) 55.57
This classifies the protein as unstable

Atomic composition
Carbon 1437
Hydrogen 2386
Nitrogen 420
Oxygen 454
Sulfur 12
Total number of atoms 4709
Formula = C1437H2386N420O454S12

Estimated half-life
mammalian reticulocytes (in vitro) 30 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 13075
assuming NO Cys residues appear as half cystines 12950

Aliphatic index
Aliphatic index 86.66

Coding Region Translation Calculations
Codon Bias 0.056
Codon Adaptation Index 0.106
Frequency of Optimal Codons 0.425
Hydropathicity of Protein -0.515
Aromaticity Score 0.041