Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
24 |
5.7% |
| Arg[R] |
20 |
4.7% |
| Asn[N] |
22 |
5.2% |
| Asp[D] |
14 |
3.3% |
| Cys[C] |
5 |
1.2% |
| Gln[Q] |
17 |
4.0% |
| Glu[E] |
26 |
6.1% |
| Gly[G] |
17 |
4.0% |
| His[H] |
9 |
2.1% |
| Ile[I] |
36 |
8.5% |
| Leu[L] |
39 |
9.2% |
| Lys[K] |
36 |
8.5% |
| Met[M] |
7 |
1.7% |
| Phe[F] |
21 |
5.0% |
| Pro[P] |
15 |
3.5% |
| Ser[S] |
41 |
9.7% |
| Thr[T] |
28 |
6.6% |
| Trp[W] |
6 |
1.4% |
| Tyr[Y] |
21 |
5.0% |
| Val[V] |
19 |
4.5% |
| Total Length |
423 |
|
| Instability index (II) |
40.29 |
| This classifies the protein as unstable |
|
| Carbon |
2204 |
| Hydrogen |
3456 |
| Nitrogen |
582 |
| Oxygen |
633 |
| Sulfur |
12 |
| Total number of atoms |
6887 |
| Formula = C2204H3456N582O633S12 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
64540 |
| assuming NO Cys residues appear as half cystines |
64290 |
| assuming all Cys residues are reduced |
64290 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
64540 M-1 cm-1 |
|
|
| Codon Bias |
-0.077 |
| Codon Adaptation Index |
0.099 |
| Frequency of Optimal Codons |
0.371 |
| Hydropathicity of Protein |
-0.316 |
| Aromaticity Score |
0.113 |
|
